A virus removal membrane is formed from cellulose, in which, when a solution containing gold colloids having a diameter of 20 nm is applied through a primary surface to the virus removal membrane to allow the virus removal membrane to capture the gold colloids for measurement of brightness in a cross section of the virus removal membrane, a value obtained by dividing a standard deviation of a value of an area of a spectrum of variation in the brightness by an average of the value of the area is 0.01 or more and 1.5 or less; and a thickness of a portion, where gold colloids having a diameter of 20 nm or more and 30 nm or less are captured, in the cross section of the virus removal membrane in a wet state is 10.0 μm or more and 30.0 μm or less.