The present disclosure provides a touch sensing structure, an operating method thereof, a touch substrate, a manufacturing method thereof, and a touch display device. The touch sensing structure includes: first signal lines; second signal lines; a plurality of sensing driving electrodes each connected to a corresponding first signal line and a corresponding second signal line; and a sensing layer in contact with the plurality of sensing driving electrodes. Rigidity of the sensing layer in contact with the sensing driving electrode changes along with a voltage difference between an electric signal applied to one of the first signal lines and an electric signal applied to the corresponding second signal line.