A thermoplastic vulcanizate is provided, including 10-50 parts by weight of a nylon plastic and 50-90 parts by weight of a cross-linked acrylic rubber polymer. The cross-linked acrylic rubber polymer is dispersed in the Nylon plastic. The cross-linked acrylic rubber polymer is formed by a modified acrylic rubber polymer having a hydrolysable silane group through crosslinking in the presence of water. In the modified acrylic rubber polymer having a hydrolysable silane group, a linkage group represented by formula (I) or formula (II) is between a backbone and the hydrolysable silane group: wherein R1 is H or —OH; R2 is H, —Z′—Si(OR′)3 or C1-C10 hydrocarbon group; R3 is H or C1-C10 hydrocarbon group; Z′ is a divalent bridging group; R′ is individually C1-C10 hydrocarbon group. A manufacturing method of the thermoplastic vulcanizate is also provided.