Invention Grant
- Patent Title: High specificity genome editing using chemically modified guide RNAs
-
Application No.: US15493129Application Date: 2017-04-20
-
Publication No.: US10767175B2Publication Date: 2020-09-08
- Inventor: Douglas J Dellinger , Daniel E Ryan , Subhadeep Roy , Jeffrey R Sampson
- Applicant: Agilent Technologies, Inc.
- Applicant Address: US CA Santa Clara
- Assignee: AGILENT TECHNOLOGIES, INC.
- Current Assignee: AGILENT TECHNOLOGIES, INC.
- Current Assignee Address: US CA Santa Clara
- Agency: Faegre Drinker Biddle & Reath LLP
- Main IPC: C12N15/11
- IPC: C12N15/11 ; C12N15/90
Abstract:
The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.
Public/Granted literature
- US20170355985A1 High Specificity Genome Editing Using Chemically Modified Guide RNAs Public/Granted day:2017-12-14
Information query
IPC分类:
