公开(公告)号：US10526590B2

公开(公告)日：2020-01-07

申请号：US15251235

申请日：2016-08-30

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Gusti Zeiner ,  Subhadeep Roy ,  Chong Wing Yung

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

公开(公告)号：US20170355985A1

公开(公告)日：2017-12-14

申请号：US15493129

申请日：2017-04-20

Applicant: Agilent Technologies, Inc.

Inventor： Douglas J. Dellinger ,  Daniel E. Ryan ,  Subhadeep Roy ,  Jeffrey R. Sampson

IPC: C12N15/11 ,  C12N15/90 ,  C12N2310/20

Abstract: The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.

公开(公告)号：US20200339980A1

公开(公告)日：2020-10-29

申请号：US16929948

申请日：2020-07-15

Applicant: Agilent Technologies, Inc.

Inventor： Douglas J Dellinger ,  Daniel E Ryan ,  Subhadeep Roy ,  Jeffrey R Sampson

IPC: C12N15/11 ,  C12N15/90

Abstract: The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.

公开(公告)号：US20170058298A1

公开(公告)日：2017-03-02

申请号：US15251235

申请日：2016-08-30

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Gusti Zeiner ,  Subhadeep Roy ,  Chong Wing Yung

IPC: C12N15/90 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3519

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

Abstract translation: 本发明涉及包含具有一个或多个修饰的衔接子部分的引导RNA，以及它们在CRISPR：Cas系统的同源重组中的用途。 修饰的衔接片段耐RNaseH降解。 本发明还涉及双指导RNA策略，其中第一引导RNA指导Cas酶在第一靶序列处进行双链断裂，并且第二引导RNA包含连接于供体多核苷酸的衔接子片段，以及 结合与第一靶序列偏移的第二靶序列。

公开(公告)号：US10767175B2

公开(公告)日：2020-09-08

申请号：US15493129

申请日：2017-04-20

Applicant: Agilent Technologies, Inc.

Inventor： Douglas J Dellinger ,  Daniel E Ryan ,  Subhadeep Roy ,  Jeffrey R Sampson

IPC: C12N15/11 ,  C12N15/90

Abstract: The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.