The present invention relates to a hydrocracking catalyst based on hierarchically porous beta-zeolite, a method of preparing the same, and a method of producing bio-jet fuel from triglyceride-containing biomass by use of the hydrocracking catalyst, and includes methods comprising preparing a hydrocracking catalyst by supporting a metallic active component on a hierarchically porous beta-zeolite support, and converting n-paraffins, produced from triglyceride-containing biomass, into bio-jet fuel by hydrocracking in the presence of the prepared hydrocracking catalyst. When the hydrocracking catalyst based on hierarchically porous beta-zeolite is used, the residence time of the reactant and the product in the zeolite crystals may be reduced due to additional mesopores formed in the zeolite, and thus bio-jet fuel may be produced in high yield from n-paraffin feedstock produced from triglyceride-containing biomass.