A method for ambient light subtraction providing a sensor having an emitter, a receiver, and a processor; performing a first sampling process, during which the emitter does not emit signals, the receiver receives a first ambient light signal converted into a first data set by the processor; performing a second sampling process, during which the emitter emits a detecting signal, the receiver receives a second ambient light signal and the detecting signal converted into a second data set by the processor; performing a third sampling process, during which the emitter does not emit signals, the receiver receives a third ambient light signal converted into a third data set by the processor; deriving an average value by calculating the average of the first data set and the third data set; and deriving a difference value by calculating the difference between the second data set and the average value.