A flexible substrate, a manufacturing method thereof and a display are provided. The method includes: forming a first organic layer on a substrate; forming a first buffer layer on the first organic layer; forming an inorganic layer on the first buffer layer; and forming a second organic layer above the inorganic layer. In this way, it is possible to enhance the adhesion between the first organic layer and the inorganic layer due to the arrangement of the first buffer layer, and in turn reduce or even eliminate the risk that the first organic layer and the inorganic layer may be fallen off from each other during the bending process of the flexible substrate, enhance the anti-water and anti-oxygen abilities of the flexible substrate, and improve the quality of the substrate.