In accordance with particular implementations of the invention described herein, a sample for analysis is illuminated under each of one or more narrow-band light sources. The light incident upon this sample is received by a sensor that generates measurement data in response thereto. One or more processors are configured to receive the measurement data and derive an excitation response curve and a fluorescent response curve from the measurement data. The processor is further configured to generate a fluorescent profile value using measurements from the fluorescent response curve for each of the captured narrow band measurement data and an excitation profile value corresponding to the area under the fluorescence curve divided by the area under the excitation curve. The generated fluorescent profile and excitation profile are both output as a dataset providing improved measurement values over similar approaches in the art.