A reference sample for analysis that is optimal for calibration of a pyrolysis gas chromatograph-mass spectrometer and with which precise calibration is always possible by preventing a reference substance from evaporating is provided. A reference sample sheet 1 is provided by distributing a target component or target components with a uniform normality in a base made of a high polymer material, and the reference sample sheet 1 is rolled up so that the target component or target components can be prevented from evaporating from the reference sample sheet 1 even in the case where a component has volatility. A reference sample for calibration of a pyrolysis gas chromatograph-mass spectrometer can be easily, quickly, and efficiently collected by punching out the reference sample sheet 1 using a micro-puncher 2.