Disclosed are a microfluid detection device, system and method, a processing device and a storage medium, in the field of biochemistry. The device includes a first substrate and a second substrate facing each other, and a microfluid chamber between the first substrate and the second substrate; wherein the first substrate has a plurality of photoelectric sensors and an output circuit, each of the photoelectric sensors is configured to convert an optical signal passing through the second substrate and the microfluid chamber to an electrical signal, and the output circuit is configured to output the electrical signal obtained by the photoelectric sensor.