Invention Grant
- Patent Title: Methods and compositions for nucleic acid amplification
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Application No.: US16308803Application Date: 2017-06-09
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Publication No.: US11268117B2Publication Date: 2022-03-08
- Inventor: Hua Yu , Theo Nikiforov , Abraham Rosenbaum
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Applicant Address: US CA Carlsbad
- Assignee: LIFE TECHNOLOGIES CORPORATION
- Current Assignee: LIFE TECHNOLOGIES CORPORATION
- Current Assignee Address: US CA Carlsbad
- International Application: PCT/US2017/036842 WO 20170609
- International Announcement: WO2017/214561 WO 20171214
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/6853 ; C12Q1/6844

Abstract:
In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5′ domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3′ domain, wherein the primer is not extendable by a polymerase, and wherein the 3′ domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.
Public/Granted literature
- US20190284597A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION Public/Granted day:2019-09-19
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