An impedance calibration circuit includes first and second calibration circuits, a switch circuit and a control circuit. The first calibration circuit is coupled to an external resistance, and generates a first voltage. The second calibration circuit generates second and third voltages. The switch circuit is coupled to the first and second calibration circuits. The switch circuit selectively provides the first, second, and third voltages to first and second nodes. The control circuit is coupled to the first and second nodes. The control circuit generates first, second, and third control signals according to voltages of the first and second nodes. In a first time interval, the switch circuit provides the first voltage to the first and second nodes. In a second time interval, the switch circuit provides the second voltage to the first and second nodes, or provides the second and third voltages respectively to the first and second nodes.