An impedance calibration circuit is provided. The impedance calibration circuit includes a first calibration circuit, a second calibration circuit and a control circuit. The first calibration circuit is adapted to be coupled to an external resistor through a calibration pad, and generate a first voltage according to a first control signal and a resistance value of the external resistor. The second calibration circuit generates a second voltage according to the first control signal and a second control signal. The control circuit is configured to compare the first voltage and a reference voltage to obtain a first comparison result, and compare the first voltage and the second voltage to obtain a second comparison result, and generate the first control signal according to the first comparison result, and generate the second control signal according to the second comparison result.