Invention Grant
- Patent Title: Separation of oligosaccharides from fermentation broth
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Application No.: US16094508Application Date: 2017-04-19
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Publication No.: US11312741B2Publication Date: 2022-04-26
- Inventor: Pierre Chassagne , Nikolay Khanzhin , Martin Matwiejuk , Markus Jondelius Hederos , Niels Banke
- Applicant: GLYCOM A/S
- Applicant Address: DK Hørsholm
- Assignee: GLYCOM A/S
- Current Assignee: GLYCOM A/S
- Current Assignee Address: DK Hørsholm
- Agency: Neal, Gerber & Eisenberg LLP
- Priority: EP16165961 20160419,EP16176257 20160624,EP16176285 20160624
- International Application: PCT/IB2017/052252 WO 20170419
- International Announcement: WO2017/182965 WO 20171026
- Main IPC: C07H13/04
- IPC: C07H13/04 ; B01D61/02 ; B01D61/14 ; B01D61/58 ; C07H1/06 ; C12P19/28 ; B01D15/36 ; B01J41/07 ; B01D61/16 ; B01J39/05 ; B01D15/18 ; B01J20/20 ; G01N30/96 ; C12P19/26 ; G01N30/02

Abstract:
The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.
Public/Granted literature
- US20190119314A1 SEPARATION OF OLIGOSACCHARIDES FROM FERMENTATION BROTH Public/Granted day:2019-04-25
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