A biomolecule analysis method including: feeding a reagent into a reaction container which includes a plurality of wells and filing the reagent in the plurality of wells, the reagent being for causing an enzymatic reaction with regard to a target substance of analysis; feeding an oil sealing solution over the plurality of wells and sealing the reagent into the plurality of wells with the oil sealing solution so that the plurality of wells become a plurality of independent reaction containers; performing the enzymatic reaction by incubating the reaction container; and detecting a signal amplified by the enzymatic reaction.