- Patent Title: Optimized gene editing utilizing a recombinant endonuclease system
-
Application No.: US15573732Application Date: 2016-05-13
-
Publication No.: US11535871B2Publication Date: 2022-12-27
- Inventor: Jason Junge , Timothy Hunt , Scott E. Fraser
- Applicant: University of Southern California
- Applicant Address: US CA Los Angeles
- Assignee: University of Southern California
- Current Assignee: University of Southern California
- Current Assignee Address: US CA Los Angeles
- Agency: Nixon Peabody LLP
- Agent Linda B. Huber
- International Application: PCT/US2016/032367 WO 20160513
- International Announcement: WO2016/183448 WO 20161117
- Main IPC: C12N15/90
- IPC: C12N15/90 ; C12N9/22 ; C12N15/85 ; C07K14/435 ; C12N15/11 ; C12N15/10

Abstract:
Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.
Public/Granted literature
- US20180127785A1 OPTIMIZED GENE EDITING UTILIZING A RECOMBINANT ENDONUCLEASE SYSTEM Public/Granted day:2018-05-10
Information query
IPC分类: