公开(公告)号：US20180127785A1

公开(公告)日：2018-05-10

申请号：US15573732

申请日：2016-05-13

Applicant: University of Southern California

Inventor： Jason Junge ,  Timothy Hunt ,  Scott E. Fraser

IPC: C12N15/90 ,  C12N9/22 ,  C07K14/435 ,  C12N15/11

CPC classification number: C12N15/907 ,  C07K14/43504 ,  C07K2319/60 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/85 ,  C12N2310/20 ,  C12N2800/80

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

公开(公告)号：US20230203541A1

公开(公告)日：2023-06-29

申请号：US18088317

申请日：2022-12-23

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Jason Junge ,  Timothy Hunt ,  Scott E. Fraser

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/85 ,  C12N15/10 ,  C07K14/435 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/85 ,  C12N15/102 ,  C07K14/43504 ,  C12N15/11 ,  C07K2319/60 ,  C07K2319/80 ,  C12N2310/20 ,  C07K2319/81 ,  C12N2800/80

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

公开(公告)号：US11535871B2

公开(公告)日：2022-12-27

申请号：US15573732

申请日：2016-05-13

Applicant: University of Southern California

Inventor： Jason Junge ,  Timothy Hunt ,  Scott E. Fraser

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/85 ,  C07K14/435 ,  C12N15/11 ,  C12N15/10

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.