Invention Grant
- Patent Title: Fluorescent multiplex cell flow systems and methods
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Application No.: US15774648Application Date: 2016-11-28
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Publication No.: US11892446B2Publication Date: 2024-02-06
- Inventor: Jasmeen Merzaban , Ayman F. Abuelela , Amal J. Ali
- Applicant: King Abdullah University of Science and Technology
- Applicant Address: SA Thuwal
- Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
- Current Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
- Current Assignee Address: SA Thuwal
- Agency: PABST PATENT GROUP LLP
- International Application: PCT/IB2016/001764 2016.11.28
- International Announcement: WO2017/089888A 2017.06.01
- Date entered country: 2018-05-09
- Main IPC: G01N33/50
- IPC: G01N33/50 ; G01N15/14 ; G01N21/05 ; G01N21/64 ; G01N15/10

Abstract:
Systems and methods are provided for simultaneously assaying cell adhesion or cell rolling for multiple cell specimens. One embodiment provides a system for assaying adhesion or cell rolling of multiple cell specimens that includes a confocal imaging system containing a parallel plate flow chamber, a pump in fluid communication with the parallel plate flow chamber via a flow chamber inlet line and a cell suspension in fluid communication with the parallel plate flow chamber via a flow chamber outlet line. The system also includes a laser scanning system in electronic communication with the confocal imaging system, and a computer in communication with the confocal imaging system and laser scanning system. In certain embodiments, the laser scanning system emits multiple electromagnetic wavelengths simultaneously it cause multiple fluorescent labels having different excitation wavelength maximums to fluoresce. The system can simultaneously capture real-time fluorescence images from at least seven cell specimens in the parallel plate flow chamber.
Public/Granted literature
- US20210311021A1 FLUORESCENT MULTIPLEX CELL FLOW SYSTEMS AND METHODS Public/Granted day:2021-10-07
Information query
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