A display substrate includes a plate and a plurality of spacers. The plate has a plate surface, and the plate surface is divided into a central region and a peripheral region, wherein the peripheral region surrounds the central region. The spacers are disposed on the plate surface in an aperiodic arrangement manner. A number of the spacers located in the central region is M, and a number of the spacers located in the peripheral region is N. An area of the central region is X, and an area of the peripheral region is Y. X, Y, M, and N satisfy the following mathematical expression: M X ≥ N Y .