A system for and method of examining a tissue sample using stimulated Raman spectroscopy is provided. The method includes: a) producing a first beam of light at a first wavelength; b) producing a second beam of light at at least a second wavelength, the second wavelength different from the first wavelength; c) combining the first and second beams of light to provide a combined output; d) interrogating a tissue sample with the combined output to produce Raman scattering light, the tissue sample prepared with at least one target molecule having a targeting agent conjugated with a Raman silent dye, the targeting agent configured to bind with at least one biomarker; e) detecting at least a portion of the produced Raman scattering light using a photodetector; and f) producing immunohistological data relating to the tissue sample using photodetector signals representative of the detected Raman scattering light.