The present invention relates to a laser scanning microscope capable of acquiring suitable fluorescent images. A fluorescence detection unit 26 measures a plurality of intensities of fluorescent light of different wavelengths that is excited by irradiation of laser light and emitted from a sample 12. Moreover, a CLEM processing unit 28 performs control, for each single pixel scanning time that is the amount of time during which a range corresponding to a single pixel of the image is scanned, to discontinue irradiation of the laser light in at least one of the case in which all of the plurality of intensities of fluorescent light are less than a lower limit threshold value at a lower limit value judgment time that is shorter than the time during which a range corresponding to a single pixel of the image is scanned, and the case in which any one of the plurality of intensities of fluorescent light exceeds an upper limit threshold value during the time during which a range corresponding to a single pixel of the image is scanned.