Invention Grant
US08227256B2 Two-color (two-photon) excitation with focused excitation beams and a raman shifter
有权
双色(双光子)激发与聚焦激发光束和一个拉曼移位器
- Patent Title: Two-color (two-photon) excitation with focused excitation beams and a raman shifter
- Patent Title (中): 双色(双光子)激发与聚焦激发光束和一个拉曼移位器
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Application No.: US10529043Application Date: 2002-09-27
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Publication No.: US08227256B2Publication Date: 2012-07-24
- Inventor: Caesar A. Saloma , Wilson O. Garcia , Jonathan A. Palero
- Applicant: Caesar A. Saloma , Wilson O. Garcia , Jonathan A. Palero
- Agency: Ohlandt, Greeley, Ruggiero & Perle, LLP
- International Application: PCT/PH02/00018 WO 20020927
- International Announcement: WO2004/029690 WO 20040408
- Main IPC: G01N21/64
- IPC: G01N21/64 ; G01N21/62 ; G01N21/63 ; G01J1/52 ; G01J3/443

Abstract:
Two-color (two-photon) excitation with two confocal excitation beams is demonstrated with a Raman shifter as excitation light source. Two-color excitation fluorescence is obtained from Coumarin 6H dye sample (peak absorption=394 nm, peak fluorescence=490 nm) that is excited using the first two Stokes outputs (683 nm, 954 nm, two-color excitation=398 nm) of a Raman shifter pumped by a 6.5 nsec pulsed 532 nm-Nd:YAG laser (Repetition rate=10 Hz). The two Stokes pulses overlap for a few nanoseconds and two-color fluorescence is generateven with focusing objectives of low numerical apertures (NA≦0.4). We observed the linear dependence of the two-color fluorescence signal with the product of the average intensities of the two Stokes excitation beams. The two-color fluorescence distribution is strongly localized around the common focus of the confocal excitation beams.
Public/Granted literature
- US20070128733A1 Two-color (two-photon) excitation with focused excitation beams and a raman shifter Public/Granted day:2007-06-07
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