Invention Grant
US08497071B2 Isolation of unknown rearranged T-cell receptors from single cells
有权
从单细胞中分离未知重排的T细胞受体
- Patent Title: Isolation of unknown rearranged T-cell receptors from single cells
- Patent Title (中): 从单细胞中分离未知重排的T细胞受体
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Application No.: US12824744Application Date: 2010-06-28
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Publication No.: US08497071B2Publication Date: 2013-07-30
- Inventor: Alejandro Benjamin Balazs , Jonathan Michael Tsai , David Baltimore
- Applicant: Alejandro Benjamin Balazs , Jonathan Michael Tsai , David Baltimore
- Applicant Address: US CA Pasadena
- Assignee: California Institute of Technology
- Current Assignee: California Institute of Technology
- Current Assignee Address: US CA Pasadena
- Agency: Knobbe, Martens, Olson & Bear LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains.
Public/Granted literature
- US20110014659A1 ISOLATION OF UNKNOWN REARRANGED T-CELL RECEPTORS FROM SINGLE CELLS Public/Granted day:2011-01-20
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