Methods and systems are described using a non-linear optical system comprising a laser and a light delivery system comprising a single mode fiber, a mode converter, and a high order mode fiber, wherein the light delivery system that receives light from the source and provides a structured free-space beam having an embedded Gaussian beam. The light delivery system functions to illuminate a region of a sample and generate a non-linear response in a spatial region smaller than that associated with a Gaussian beam having a width comparable to the width of the embedded Gaussian beam. In another aspect, the light delivery system illuminates a region of a sample and generates a non-linear emission of radiation, is depicted. A further aspect of this embodiment includes an imaging assembly for detecting the non-linear emission and using a signal derived from the detected emission to generate a microscopic image of the sample.