Invention Grant
US08525104B2 Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry
有权
通过基质辅助激光解吸电离（MALDI）质谱法进行直接生物分子鉴定的方法
- Patent Title: Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry
- Patent Title (中): 通过基质辅助激光解吸电离（MALDI）质谱法进行直接生物分子鉴定的方法
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Application No.: US12799576Application Date: 2010-04-27
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Publication No.: US08525104B2Publication Date: 2013-09-03
- Inventor: Paul Pevsner , Frederick Naftolin , Douglas C. Miller , Dean Hillman , Brian K. Stall , Steven M. Wishnies
- Applicant: Paul Pevsner , Frederick Naftolin , Douglas C. Miller , Dean Hillman , Brian K. Stall , Steven M. Wishnies
- Applicant Address: US NY New York
- Assignee: New York University
- Current Assignee: New York University
- Current Assignee Address: US NY New York
- Agency: Klauber & Jackson LLC
- Main IPC: B01D59/44
- IPC: B01D59/44 ; H01J49/00 ; H01J49/40
Abstract:
The present invention relates to the use of post source decay (PSD) or collision induced dissociation (CID) direct tissue (DT) MALDI-TOF or DT-MALDI-TOF-TOF mass spectrographic identification of biological molecules in a tissue or cellular sample without the need for further protein extraction. This method provides for studying cells or tissues by direct tissue MALDI (DT-MALDI), thereby substituting in situ protein release for further protein extraction. Mass/intensity data was processed with Mascot© software interrogation of the NCBI database. These results are proof of principle that DT-MALDI, combined with bioinformatics, can directly identify proteins in cells and tissues from their mass spectra.
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