Invention Grant
US08586945B2 Fluorescence light scanning microscope having a birefringent chromatic beam shaping device
有权
具有双折射彩色光束整形装置的荧光光扫描显微镜
- Patent Title: Fluorescence light scanning microscope having a birefringent chromatic beam shaping device
- Patent Title (中): 具有双折射彩色光束整形装置的荧光光扫描显微镜
-
Application No.: US13297872Application Date: 2011-11-16
-
Publication No.: US08586945B2Publication Date: 2013-11-19
- Inventor: Matthias Reuss , Johann Engelhardt
- Applicant: Matthias Reuss , Johann Engelhardt
- Applicant Address: DE Heidelberg
- Assignee: Deutsches Krebsforschungszentrum
- Current Assignee: Deutsches Krebsforschungszentrum
- Current Assignee Address: DE Heidelberg
- Agency: Thomas | Horstemeyer, LLP
- Priority: DE202009007250U 20090520
- Main IPC: G01J1/58
- IPC: G01J1/58

Abstract:
A fluorescence light scanning microscope (2) comprises a light source providing excitation light (8) for exciting a fluorophore in a sample to be imaged for spontaneous emission of fluorescence light, and suppression light (7) for suppressing spontaneous emission of fluorescence light by the fluorophore on a common optical axis (4), the suppression wavelength differing from the excitation wavelength; an objective (19) focusing both the excitation (8) and the suppression (7) light to a focus point; a detector (21) detecting fluorescence light (11) spontaneously emitted by the fluorophore; and a chromatic beam shaping device (1) arranged on the common optical axis (4), and including a birefringent chromatic optical element (3) adapted to shape a polarization distribution of the suppression light (7) such as to produce an intensity zero at the focus point, and to leave the excitation light such as to produce a maximum at the focus point.
Public/Granted literature
- US20120104279A1 Fluorescence Light Scanning Microscope Having a Birefringent Chromatic Beam Shaping Device Public/Granted day:2012-05-03
Information query