Invention Grant
- Patent Title: Ribonucleic acid identification apparatus, ribonucleic acid identification method, program and ribonucleic acid identification system
- Patent Title (中): 核糖核酸鉴定仪,核糖核酸鉴定方法,程序和核糖核酸鉴定系统
-
Application No.: US12988037Application Date: 2009-04-17
-
Publication No.: US08880353B2Publication Date: 2014-11-04
- Inventor: Hiroshi Nakayama , Misaki Akiyama , Toshiaki Isobe , Masato Taoka , Yoshio Yamauchi , Nobuhiro Takahashi , Hideaki Ishikawa
- Applicant: Hiroshi Nakayama , Misaki Akiyama , Toshiaki Isobe , Masato Taoka , Yoshio Yamauchi , Nobuhiro Takahashi , Hideaki Ishikawa
- Applicant Address: JP Wako-shi JP Tokyo JP Tokyo
- Assignee: Riken,Tokyo Metropolitan University,National University Corporation Tokyo University of Agriculture and Technology
- Current Assignee: Riken,Tokyo Metropolitan University,National University Corporation Tokyo University of Agriculture and Technology
- Current Assignee Address: JP Wako-shi JP Tokyo JP Tokyo
- Agency: Foley & Lardner LLP
- Priority: JP2008-108369 20080417
- International Application: PCT/JP2009/057739 WO 20090417
- International Announcement: WO2009/128526 WO 20091022
- Main IPC: G01N33/48
- IPC: G01N33/48 ; C12Q1/68 ; H01J49/00

Abstract:
Disclosed are an apparatus for the identification of a ribonucleic acid, whereby not only the molecular weights of digestion products, from which nothing but the nucleic acid residue composition can be understood, but also nucleic acid residue sequence data is obtained from a product ion mass set and thus the identification reliability for the individual digestion products can be remarkably improved; a method for the identification of a ribonucleic acid; and a program and system for the identification of a ribonucleic acid. The method as described above comprises: searching for nucleic acid sequence database by using a fragment mass set indicating the molecular weights and inner structural data of individual oligonucleotides that are obtained by tandem mass spectrometry on digested oligonucleotides formed by chemically or enzymatically cleaving a ribonucleic acid; scoring candidate sequences; among candidate sequences showing the highest scores, referring those showing scores exceeding the threshold as identified sequences; and using these identified sequences in mapping on nucleic acid sequence database including genomic sequences.
Public/Granted literature
Information query