Invention Grant
- Patent Title: Sphingomyelin measurement method using sequential phospholipase D reactions
- Patent Title (中): 鞘磷脂测定方法采用顺序磷脂酶D反应
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Application No.: US14232427Application Date: 2012-07-25
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Publication No.: US09051600B2Publication Date: 2015-06-09
- Inventor: Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
- Applicant: Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
- Applicant Address: JP Tokyo
- Assignee: KYOWA MEDEX CO., LTD.
- Current Assignee: KYOWA MEDEX CO., LTD.
- Current Assignee Address: JP Tokyo
- Agency: Fitzpatrick, Cella, Harper & Scinto
- Priority: JP2011-166458 20110729
- International Application: PCT/JP2012/068807 WO 20120725
- International Announcement: WO2013/018609 WO 20130207
- Main IPC: C12Q1/44
- IPC: C12Q1/44 ; C12Q1/61 ; G01N33/92 ; C12Q1/28
Abstract:
Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
Public/Granted literature
- US20140162300A1 SPHINGOMYELIN MEASUREMENT METHOD AND MEASUREMENT KIT Public/Granted day:2014-06-12
Information query
