A waterproof gate having a speaker mesh film is provided and applied to a case, and the case is formed with a slot. The waterproof gate includes that a waterproof unit is plugged in the slot and has a partition plate having two opposite surfaces being respectively extended with a first annular piece and a second annular piece, and the partition plate is formed with an opening; a speaker mesh film unit covers the opening; a first fasten member is disposed and fastened in a first recess defined by the first annular piece and the partition plate; and a second fasten member is disposed and fastened in a second recess defined by the second annular piece and the partition plate. The speaker mesh film unit and the partition plate are clamped between the first fasten member and the second fasten member. Accordingly, an excellent waterproof effect is provided.