An image analysis method includes acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. The method further includes setting analysis areas to the fluorescent images, selecting the fluorescent images of two or more frames to be used in analysis, extracting data pairs each comprising two pixels in which acquisition time intervals are the same in the analysis area of each of the selected fluorescent images, and performing product sum calculation of each of the data pairs for all of the selected images to calculate a correlation value.