Invention Grant
US09399768B2 Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
有权
在脂肪酸合成中使用酰基酰基载体蛋白硫酯酶及其突变体和嵌合体的材料和方法
- Patent Title: Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
- Patent Title (中): 在脂肪酸合成中使用酰基酰基载体蛋白硫酯酶及其突变体和嵌合体的材料和方法
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Application No.: US14141327Application Date: 2013-12-26
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Publication No.: US09399768B2Publication Date: 2016-07-26
- Inventor: Basil J. Nikolau , Marna Yandeau-Nelson , Fuyuan Jing
- Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
- Applicant Address: US IA Ames
- Assignee: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
- Current Assignee: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
- Current Assignee Address: US IA Ames
- Agency: Larcher & Chao Law Group
- Agent Carol Larcher
- Main IPC: C12N9/16
- IPC: C12N9/16 ; C12N1/20 ; C12N1/12 ; C07H21/04 ; C12P7/64
Abstract:
A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.
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