An image sensor is provided. The image sensor includes a pixel array, a sense amplifier, an analog-to-digital converter, a processor and a voltage generator. The sense amplifier obtains a first signal according to a pixel signal from the pixel array and a reference voltage, wherein the reference voltage has a first voltage level. The analog-to-digital converter converts the first signal into a first digital signal. The processor provides a feedback signal according to the first digital signal. The voltage generator adjusts the reference voltage to a second voltage level corresponding to the feedback signal. The sense amplifier removes a direct current (DC) bias voltage from the pixel signal according to the reference signal having the second voltage level.