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US09502227B2 Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis 有权
在细胞和仪器观察下细胞液的捕获和其组分的分析，用于细胞液捕获和分析

Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis
Abstract:
A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.
Abstract(Chinese):

一种方法从单个细胞捕获细胞组分，并对组分进行质谱分析。 该方法包括用显微镜将纳喷射电离毛细管尖端插入细胞的特定区域。 纳米喷雾电离毛细管尖端可以在内部包括细丝。 该方法还包括将细胞的特定区域的细胞组分捕获到纳喷射电离毛细管尖端的开口中，并将组分保持在纳喷射离子化毛细管尖端处，从纳滤喷射离子化毛细管的后端提供电离支持溶剂 尖端，在质谱仪的样品入口和纳喷射电离毛细管尖端之间施加电场，由此实现对细胞组分的纳米喷雾电离，并对在纳米喷雾电离毛细管尖端捕获的细胞组分进行质谱分析。

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