Invention Grant
US09557253B2 Electron microscopic observation method for observing biological sample in shape as it is, and composition for evaporation suppression under vacuum, scanning electron microscope, and transmission electron microscope used in the method
有权
用于观察生物样品形状的电子显微镜观察方法，以及该方法中使用的真空蒸发抑制组合物，扫描电子显微镜和透射电子显微镜
- Patent Title: Electron microscopic observation method for observing biological sample in shape as it is, and composition for evaporation suppression under vacuum, scanning electron microscope, and transmission electron microscope used in the method
- Patent Title (中): 用于观察生物样品形状的电子显微镜观察方法，以及该方法中使用的真空蒸发抑制组合物，扫描电子显微镜和透射电子显微镜
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Application No.: US14343537Application Date: 2012-09-07
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Publication No.: US09557253B2Publication Date: 2017-01-31
- Inventor: Takahiko Hariyama , Yasuharu Takaku , Hiroshi Suzuki , Yoshinori Muranaka , Isao Ohta , Masatsugu Shimomura , Daisuke Ishii
- Applicant: Takahiko Hariyama , Yasuharu Takaku , Hiroshi Suzuki , Yoshinori Muranaka , Isao Ohta , Masatsugu Shimomura , Daisuke Ishii
- Applicant Address: JP Saitama
- Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
- Current Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
- Current Assignee Address: JP Saitama
- Agency: Wenderoth, Lind & Ponack, L.L.P.
- Priority: JP2011-197685 20110909; JP2012044383 20120229
- International Application: PCT/JP2012/072982 WO 20120907
- International Announcement: WO2013/035866 WO 20130314
- Main IPC: H01J37/00
- IPC: H01J37/00 ; G01N1/44 ; G01N23/225 ; G01N23/04 ; H01J37/20 ; G01N1/28
Abstract:
Provided is an observation method by an electron microscope, in which a biological sample can be observed as it is alive and a situation that the biological sample is moving can be observed using an electron microscope, and a composition for evaporation suppression under vacuum, a scanning electron microscope, and a transmission electron microscope used in the method.The sample observation method by an electron microscope according to the invention includes applying a composition for evaporation suppression containing at least one kind selected from an amphiphilic compound, oils and fats, and an ionic liquid to the surface of a sample to form a thin film, and covering the sample with the thin film, and displaying an electron microscopic image of the sample, which is covered with the thin film and accommodated in a sample chamber under vacuum, on a display device.
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