Invention Grant
- Patent Title: Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
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Application No.: US14378268Application Date: 2013-02-14
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Publication No.: US09598728B2Publication Date: 2017-03-21
- Inventor: Francis Barany , Eugene Spier , Alain Mir
- Applicant: CORNELL UNIVERSITY
- Applicant Address: US NY Ithaca
- Assignee: Cornell University
- Current Assignee: Cornell University
- Current Assignee Address: US NY Ithaca
- Agency: LeClairyRyan, a Professional Corporation
- International Application: PCT/US2013/026180 WO 20130214
- International Announcement: WO2013/123220 WO 20130822
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.
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