A fiber optic probe assembly is provided. The probe comprises a first optical system and a second optical system, a delivery light guide comprising one or more than one delivery optical fiber for transmitting excitation radiation from a radiation source disposed at a proximal end of the light guide to the first optical system. The first optical system comprising one or more than one first optical element for forming a substantially collimated illumination beam from the excitation radiation. An optically opaque tubular sleeve is fitted over the first optical system to optically isolate the first optical system and the delivery light guide from the second optical system. The second optical system comprising one or more than one second optical element for gathering optical radiation scattered from a sample and forming the optical radiation into a collection beam. A collection light guide comprising one or more than one collection optical fiber receives the collection beam and transmits the collection beam to an analyzer. The first and second optical systems are disposed within a housing so that an emission cone of the first optical system and an acceptance cone of the second optical system substantially overlap. A spectroscopic measurement system comprising the optic fiber probe is also provided.