A method of manufacturing a color conversion substrate includes preparing a substrate including a first pixel region, a second pixel region, and a third pixel region, forming a first color conversion portion corresponding to the first pixel region, forming a second color conversion portion corresponding to the second pixel region, and forming a third color conversion portion corresponding to the third pixel region. The providing the first color conversion portion includes providing a first color filter of a first color corresponding to the first pixel region, providing a first transparent pattern corresponding to the first pixel region, and providing a first pattern layer corresponding to the first pixel region and including a first material which converts light incident thereto into light of the first color, where the first transparent pattern is provided between the first color filter and the first pattern layer.