A replacement glove assembly has a replacement access element defining an outermost shoulder end and an innermost hand end configured to receive a hand of a user. A replacement sealing assembly engages the outermost shoulder end of the replacement access element and defines a leading radial face defining a first coupling structure, a trailing radial face defining a second coupling structure, and an outer annular surface configured to be received by a glove port. A first glove cap has a first side and a second side opposite the first side. The first side defines a first mating structure sealably engaged with the first coupling structure of the leading radial face. The second side defines a second mating structure that is configured to sealably engage the second coupling structure of the trailing radial face of the replacement sealing assembly.