Invention Grant
- Patent Title: Biomolecular processing platform and uses thereof
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Application No.: US14763972Application Date: 2014-02-10
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Publication No.: US09909173B2Publication Date: 2018-03-06
- Inventor: Steven A. Soper , Francis Barany , George Grills , Robin McCarley , Collin J. McKinney , Dorel Moldovan , Michael C. Murphy , Dimitris Nikitopoulos , Sunggook Park , Elizabeth J. Podlaha-Murphy
- Applicant: Cornell University , Board of Supervisors of Louisiana State University and Agricultural and Mechanical College , University of North Carolina at Chapel Hill , Northeastern University
- Applicant Address: US NY Ithaca US NC Chapel Hill US MA Boston US LA Baton Rouge
- Assignee: Cornell University,University of North Carolina at Chapel Hill,Northeastern University,Board of Supervisors of Louisiana State University and Agriculture and Mechanical College
- Current Assignee: Cornell University,University of North Carolina at Chapel Hill,Northeastern University,Board of Supervisors of Louisiana State University and Agriculture and Mechanical College
- Current Assignee Address: US NY Ithaca US NC Chapel Hill US MA Boston US LA Baton Rouge
- Agency: LeClairRyan, a Professional Corporation
- International Application: PCT/US2014/015574 WO 20140210
- International Announcement: WO2014/124365 WO 20140814
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; B01L3/00 ; G01N27/447 ; G01N33/68 ; H01J49/00 ; G01N33/487

Abstract:
The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor contacting the time-of-flight channel proximate to the input end of the channel and (ii) a second sensor contacting the time-of-flight channel proximate to the output end of channel. The present invention further relates to methods of sequencing and identifying biopolymer molecules using the device.
Public/Granted literature
- US20150361489A1 BIOMOLECULAR PROCESSING PLATFORM AND USES THEREOF Public/Granted day:2015-12-17
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