公开(公告)号：KR101311272B1

公开(公告)日：2013-09-27

申请号：KR1020110023509

申请日：2011-03-16

Applicant: 고려대학교 산학협력단

Inventor： 최인걸 ,  김경헌 ,  고혁진

IPC: C12N15/63 ,  C12N15/70 ,  C12N15/31

Abstract: 본 발명은 오토트랜스포터 단백질을 이용한 세포표면 발현 시스템의 구축에 관한 발명이다.

公开(公告)号：KR1020120077339A

公开(公告)日：2012-07-10

申请号：KR1020100139266

申请日：2010-12-30

Applicant: 고려대학교 산학협력단

Inventor： 최인걸 ,  김경헌 ,  고혁진 ,  김소현

IPC: C12N15/74 ,  C12N15/65 ,  C12N15/31

Abstract: PURPOSE: A method for transducing a gene into Saccharophagus degradans and overexpressing the gene is provided to enable industrial application for producing bioethanol. CONSTITUTION: A method for expressing a gene in Saccharophagus degradans comprises: a step of injecting a vector structure containing a promoter into the cells of Saccharophagus degradans; and a step of overexpressing the gene. The promoter is aga 16B promoter. The vector structure further contains the origin of replication, such as pBBR1 or pMB1. The vector structure also contains a recombinant DNA selection marker. The marker is a kanamycin, gentamycin, or Chlorampenychole resistant gene.

Abstract translation: 目的：提供一种将基因转导入酵母属降解物并过量表达该基因的方法，以使工业化生产生物乙醇。 构成：在酿酒酵母降解体中表达基因的方法包括：将含有启动子的载体结构注射到酵母属分解菌的细胞中的步骤; 以及过表达基因的步骤。 启动子是aga 16B启动子。 载体结构还包含复制起点，如pBBR1或pMB1。 载体结构还含有重组DNA选择标记。 标记物是卡那霉素，庆大霉素或氯安宁抗性基因。

公开(公告)号：KR101322797B1

公开(公告)日：2013-10-29

申请号：KR1020100139266

申请日：2010-12-30

Applicant: 고려대학교 산학협력단

Inventor： 최인걸 ,  김경헌 ,  고혁진 ,  김소현

IPC: C12N15/74 ,  C12N15/65 ,  C12N15/31

Abstract: 본 발명은 사카로파거스 데그라단스(Saccharophagus degradans) 세포 내로 유전자를 도입하고, 사카로파거스 데그라단스에서 유전자를 과다 발현시키는 방법 및 그 응용에 관한 것이다.

公开(公告)号：KR1020120106911A

公开(公告)日：2012-09-27

申请号：KR1020110023509

申请日：2011-03-16

Applicant: 고려대학교 산학협력단

Inventor： 최인걸 ,  김경헌 ,  고혁진

IPC: C12N15/63 ,  C12N15/70 ,  C12N15/31

Abstract: PURPOSE: A method for establishing a cell surface expression system using an autotransporter is provided to enable secretion of target proteins. CONSTITUTION: A surface expression vector contains a signal sequence, a passenger domain, a linker site, and a cloning site in which the passenger domain site is deleted from autotransporter proteins of bacteria. A vector contains TEV protease cleavage site inserted to the cloning site. The autotransporter protein has an amino acid sequence of sequence number 1. The passenger domain site has a sequence of 29th to 785th residue in an amino acid of sequence number 1. [Reference numerals] (AA) Cleavable leader sequence; (BB) Hypothetic linker site; (CC) Passenger domain with adhering function; (DD) Pertacin-like domain; (EE) Autotransporter series; (FF) N-terminal passenger domain; (GG) C-terminal passenger domain; (HH) Removal site

Abstract translation: 目的：提供使用自身转运蛋白建立细胞表面表达系统的方法，以使靶蛋白分泌。 构成：表面表达载体含有信号序列，乘客结构域，接头位点和克隆位点，其中乘客域位点从细菌的自身转运蛋白中缺失。 载体含有插入克隆位点的TEV蛋白酶切割位点。 自身转运蛋白具有序列号1的氨基酸序列。载体结构域位点在序列号1的氨基酸中具有29至785位残基的序列。[参考数字]（AA）可剪切前导序列; （BB）虚拟连接位点; （CC）具有粘性功能的客运区; （DD）Pertacin样结构域; （EE）自动输送机系列; （FF）N端乘客域; （GG）C终端乘客区域; （HH）搬迁站点