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公开(公告)号:KR1020120077339A
公开(公告)日:2012-07-10
申请号:KR1020100139266
申请日:2010-12-30
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for transducing a gene into Saccharophagus degradans and overexpressing the gene is provided to enable industrial application for producing bioethanol. CONSTITUTION: A method for expressing a gene in Saccharophagus degradans comprises: a step of injecting a vector structure containing a promoter into the cells of Saccharophagus degradans; and a step of overexpressing the gene. The promoter is aga 16B promoter. The vector structure further contains the origin of replication, such as pBBR1 or pMB1. The vector structure also contains a recombinant DNA selection marker. The marker is a kanamycin, gentamycin, or Chlorampenychole resistant gene.
Abstract translation: 目的:提供一种将基因转导入酵母属降解物并过量表达该基因的方法,以使工业化生产生物乙醇。 构成:在酿酒酵母降解体中表达基因的方法包括:将含有启动子的载体结构注射到酵母属分解菌的细胞中的步骤; 以及过表达基因的步骤。 启动子是aga 16B启动子。 载体结构还包含复制起点,如pBBR1或pMB1。 载体结构还含有重组DNA选择标记。 标记物是卡那霉素,庆大霉素或氯安宁抗性基因。
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