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公开(公告)号：KR1020130095868A
公开(公告)日：2013-08-29
申请号：KR1020120017196
申请日：2012-02-21
Applicant: 국민대학교산학협력단
Abstract: PURPOSE: A screening method of regulating agents of BAK protein channel formation is provided to search apoptosis suppressing agents or apoptosis promoting agents, thereby being useful to develop drugs for a stroke, degenerative brain diseases, or cancers. CONSTITUTION: A composition for screening regulating agents of BAK protein channel formation comprises: BID protein; BAK protein; and liposome in which a fluorescent material is sampled. A screening method of regulating agents of BAK protein channel formation comprises: a step of mixing activated BID protein and BAK protein, in which histidine-tag (His-tag) is attached, in buffer solution; a step of treating the regulating agents of BAK protein channel formation to the mixture; a step of reacting by adding the liposome, in which the fluorescent material is sampled and the histidine-tag (His-tag) is attached, to the workpiece; a step of measuring a fluorescence signal of the reaction product; and a step of comparing the measured value between the above fluorescence signal and which of the control group in which BAK protein channel formation regulating candidate is not treated.
Abstract translation: 目的：提供BAK蛋白通道形成调节剂的筛选方法，以寻找凋亡抑制剂或细胞凋亡促进剂，从而可用于开发中风，退行性脑病或癌症药物。 构成：用于筛选BAK蛋白通道形成调节剂的组合物包括：BID蛋白; BAK蛋白; 和其中对荧光材料进行取样的脂质体。 BAK蛋白通道形成调节剂的筛选方法包括：将缓冲溶液中附着有组氨酸标签（His标签）的活化BID蛋白和BAK蛋白质混合的步骤; 将BAK蛋白通道形成调节剂处理至混合物的步骤; 通过将其中附着有荧光材料的脂质体和附着有组氨酸标签（His标签）的反应添加到工件中的步骤; 测定反应产物的荧光信号的步骤; 以及比较上述荧光信号与未处理BAK蛋白通道形成调节候选物的对照组中的哪一个之间的测定值的步骤。
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公开(公告)号：KR101344716B1
公开(公告)日：2013-12-26
申请号：KR1020120017196
申请日：2012-02-21
Applicant: 국민대학교산학협력단
Abstract: 본발명은 BAK 단백질의채널형성조절물질(channel formation regulating agents) 스크리닝용조성물, 이를포함하는스크리닝용키트(kit), 및이를이용한스크리닝방법에관한것이다. 본발명은세포사멸신호에의하여활성화되는 BAK 채널형성의정도를측정함으로써세포사멸을억제하는물질또는세포사멸이원활하게이루어지도록촉진하는물질을탐색하여뇌졸중, 퇴행성뇌질환또는항암제개발에유용하게이용될수 있다.
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