公开(公告)号：KR100433280B1

公开(公告)日：2004-05-31

申请号：KR1020000055634

申请日：2000-09-22

Applicant: 대한민국(충남대학교)

Inventor： 박창식 ,  이영주 ,  임기순 ,  천용민

IPC: A01N1/00

Abstract: PURPOSE: A diluent for freezing boar sperm obtained by adding N-acetyl-D-glucosamine as water soluble chitin to boar sperm, freezing and then thawing is provided which improves viability and movability of boar sperm and can be applied to cow, chicken or other domestic animals. CONSTITUTION: This diluent comprises 11g lactose hydrate, 20ml egg yolk, 4% by weight of glycerol, 1.0% by weight of orvus es paste(OEP) and 0 to 0.05g N-acetyl-D-glucosamine, based on 100ml diluent. A sperm-rich fraction separated from livestock is subjected to cooling slowly at room temperature for 90 to 150 min and then centrifugal separation at 1,500rpm for 5 to 15 min. The pretreated sperm is added with 5.5ml first diluent composing 11g lactose hydrate, 20ml egg yolk, 0.01 to 1.0g N-acetyl-D-glucosamine and 100ml distilled water and then left alone at 4 to 6deg.C for 90 to 150 min. The first sperm liquid is diluted with 11ml second dilute comprising 11g lactose hydrate, 20ml egg yolk, 4% by weight of glycerol, 1.0% by weight of OEP, 0.01 to 1.5g N-acetyl-D-glucosamine and distilled water and then subjected to glycerol equilibrium.

Abstract translation: 用途：通过将N-乙酰-D-氨基葡萄糖作为水溶性几丁质添加到公猪精液中，冷冻然后解冻而得到的用于冷冻公猪精液的稀释剂，其提供公猪精子的活力和活动性，并且可以应用于牛，鸡或其他动物 家养动物。 构成：基于100ml稀释剂，该稀释剂包含11g乳糖水合物，20ml蛋黄，4重量％甘油，1.0重量％环糊精（OEP）和0-0.05g N-乙酰基-D-葡糖胺。 将从家畜分离的精子富集部分在室温下缓慢冷却90至150分钟，然后在1,500rpm下离心分离5至15分钟。 预先处理的精子加入5.5ml第一稀释液，组成11g乳糖水合物，20ml蛋黄，0.01-1.0g N-乙酰-D-氨基葡萄糖和100ml蒸馏水，然后在4-6℃下放置90-150分钟。 用11ml第二稀释液稀释第一精子液，该稀释液包含11g乳糖水合物，20ml蛋黄，4重量％甘油，1.0重量％OEP，0.01-1.5g N-乙酰基-D-葡糖胺和蒸馏水，然后进行 达到甘油平衡。

公开(公告)号：KR1020020023001A

公开(公告)日：2002-03-28

申请号：KR1020000055634

申请日：2000-09-22

Applicant: 대한민국(충남대학교)

Inventor： 박창식 ,  이영주 ,  임기순 ,  천용민

IPC: A01N1/00

CPC classification number: A01N1/0221 ,  C12N5/061

Abstract: PURPOSE: A diluent for freezing boar sperm obtained by adding N-acetyl-D-glucosamine as water soluble chitin to boar sperm, freezing and then thawing is provided which improves viability and movability of boar sperm and can be applied to cow, chicken or other domestic animals. CONSTITUTION: This diluent comprises 11g lactose hydrate, 20ml egg yolk, 4% by weight of glycerol, 1.0% by weight of orvus es paste(OEP) and 0 to 0.05g N-acetyl-D-glucosamine, based on 100ml diluent. A sperm-rich fraction separated from livestock is subjected to cooling slowly at room temperature for 90 to 150 min and then centrifugal separation at 1,500rpm for 5 to 15 min. The pretreated sperm is added with 5.5ml first diluent composing 11g lactose hydrate, 20ml egg yolk, 0.01 to 1.0g N-acetyl-D-glucosamine and 100ml distilled water and then left alone at 4 to 6deg.C for 90 to 150 min. The first sperm liquid is diluted with 11ml second dilute comprising 11g lactose hydrate, 20ml egg yolk, 4% by weight of glycerol, 1.0% by weight of OEP, 0.01 to 1.5g N-acetyl-D-glucosamine and distilled water and then subjected to glycerol equilibrium.

Abstract translation: 目的：提供通过将N-acetyl-D-glucosamine作为水溶性几丁质加入到野猪精液中，冷冻，然后解冻而获得的用于冷冻野猪精子的稀释剂，其提高了公猪精子的活力和可移动性，并且可以应用于牛，鸡或其他 家畜。 构成：该稀释剂基于100ml稀释剂，包含11g乳糖水合物，20ml蛋黄，4％（重量）甘油，1.0％（重量）orvus es糊剂（OEP）和0至0.05g N-乙酰基-D-葡糖胺。 将与家畜分离的富含精子的级分在室温下缓慢冷却90至150分钟，然后以1500rpm离心分离5至15分钟。 加入预先处理的精子，加入组成11g乳糖水合物，20ml蛋黄，0.01〜1.0g N-乙酰基-D-葡萄糖胺和100ml蒸馏水的5.5ml第一稀释液，然后在4〜6℃下静置90〜150分钟。 将第一精子液稀释为11ml第二稀释液，其包含11g乳糖水合物，20ml蛋黄，4wt％甘油，1.0wt％OEP，0.01-1.5g N-乙酰基-D-葡糖胺和蒸馏水，然后 到甘油平衡。