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    신규한 토양 미생물, 상기 토양 미생물로부터 분리된신규한 베타트랜스아미나제, 상기 베타트랜스아미나제를암호화하는 유전자 및 이들을 이용하여 광학적으로 순수한베타아미노산과 그 유도체를 생산하는 방법
    1.
    发明公开
    신규한 토양 미생물, 상기 토양 미생물로부터 분리된신규한 베타트랜스아미나제, 상기 베타트랜스아미나제를암호화하는 유전자 및 이들을 이용하여 광학적으로 순수한베타아미노산과 그 유도체를 생산하는 방법 有权
    一种新型的土壤微生物，从土壤微生物分离的新颖的BETA透明质酸酶，编码BETA转移酶的基因，以及使用它们生产高效纯氨酸氨基酸及其衍生物的方法

    公开(公告)号：KR1020080016287A

    公开(公告)日：2008-02-21

    申请号：KR1020060078204

    申请日：2006-08-18

    Applicant: 재단법인서울대학교산학협력재단

    Inventor： 김병기 ,  김주한 ,  경도현 ,  윤형돈

    IPC: C12N1/20 ,  C12P21/00

    Abstract: A novel microorganism primarily isolated from soil using a minimum culture medium including a beta amino acid as a single nitrogen source is provided to show the high optical selectivity and transaminase activity on a D-type beta amino acid. And a method for producing an L-type or a D-type beta amino acid is provided to be able to obtain the enantiomerically pure product using the same microorganism. A Mesorhizobium sp. LUK is deposited as a deposition no. KCCM-10752P. An L-type beta amino acid is produced at a temperature of 20-70 deg.C under pH of 5-10 by using the Mesorhizobium sp. LUK or a cell extract thereof as a bio-catalyst through optical resolution. A D-type beta amino acid is produced at a temperature of 20-70 deg.C under pH of 5-10 by using the Mesorhizobium sp. LUK or the cell extract thereof as a bio-catalyst through asymmetric synthesis. A transformed host cell is transformed by a recombinant DNA vector including a beta-transaminase gene encoding an amino acid sequence of SEQ ID : NO. 3 or a gene having more than 97% homology to the sequence of SEQ ID : NO. 3 and encoding a protein with the beta-transaminase activity. Further, an amino accepter for the optical resolution is selected from keto acid group.

    Abstract translation: 提供了使用包含β氨基酸作为单一氮源的最小培养基从土壤中主要分离的新型微生物，以显示对D型β氨基酸的高光学选择性和转氨酶活性。 并且提供了用于生产L型或D型β氨基酸的方法以能够使用相同的微生物获得对映异构体纯的产物。 中生根瘤菌 LUK作为沉积物进行沉积。 KCCM-10752P。 通过使用中生根瘤菌属（Mesorhizobium sp。），在pH 5-10的温度下，在20-70℃的温度下产生L型β-氨基酸。 LUK或其细胞提取物作为生物催化剂通过光学拆分。 通过使用中生根瘤菌属（Mesorhizobium sp。），在pH 5-10的温度下，在20-70℃的温度下产生D型β氨基酸。 LUK或其细胞提取物作为通过不对称合成的生物催化剂。 通过重组DNA载体转化转化的宿主细胞，所述重组DNA载体包含编码SEQ ID NO：1的氨基酸序列的β-转氨酶基因。 3或与SEQ ID NO：3的序列具有超过97％同源性的基因。 3并编码具有β-转氨酶活性的蛋白质。 此外，用于光学拆分的氨基取代基选自酮酸基团。

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