公开(公告)号：KR100824218B1

公开(公告)日：2008-04-25

申请号：KR1020060128318

申请日：2006-12-15

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이병천 ,  황우석 ,  강성근 ,  김민규 ,  장구 ,  오현주 ,  유다헤루 ,  김혜진 ,  김정주 ,  신남식 ,  후세인샤밍

IPC: C12N5/26 ,  C12N5/07

Abstract: A method for producing a cloned wolf is provided to contribute to preserve a rare and endangered species wolf and develop veterinary medicine, anthropology and medicine study fields. A method for preparing a nuclear transplant embryo of wolf comprises the steps of: (a) removing a nucleus from ovum of a canine animal such as dog, wolf, fox, jackal, coyote, dhole, and raccoon dog to prepare a denucleated ovum; (b) isolating a somatic cell from a tissue of the wolf to prepare a donor nucleus cell; (c) micro-injecting the donor nucleus cell into the denucleated ovum and electrofusing it; and (d) activating the fused ovum. A nuclear transplant embryo prepared by the method is deposited as a deposition no. KCTC 10903BP. A method for producing a cloned wolf comprises a step of transplanting the nuclear transplant embryo into an oviduct of a canine animal surrogate mother. Further, the electrofusing step is performed at a voltage of 3.6 to 4.1 kv/cm, for 10 to 30 mus, 1 to 3 times.

Abstract translation: 提供了克隆狼的生产方法，有助于保护珍稀濒危物种狼，开发兽药，人类学和医学研究领域。 制备狼的核移植胚胎的方法包括以下步骤：（a）从狗，狼，狐狸，豺狼，土拨鼠，hole和浣熊犬等动物的卵中除去核，以制备脱核卵; （b）从狼的组织中分离体细胞以制备供体核细胞; （c）将供体核细胞微注射到去核的卵中并将其电铸; 和（d）激活融合的卵子。 通过该方法制备的核移植胚胎被沉积为沉积物。 KCTC 10903BP。 克隆的狼的制备方法包括将核移植胚胎移植到犬动物替代母亲的输卵管中的步骤。 此外，电熔步骤在3.6至4.1kv / cm的电压下进行10至30次，1至3次。