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Patent Agency Ranking1.发明公开서머스 플라버스(Ｔｈｅｒｍｕｓ ｆｌａｖｕｓ) ＡＴ-62유래 신규의 내열성 베타-글리코시다제 ｔａｔ β-ｇｌｙ 无效从THERMUS FLAVUS AT-62衍生的新型可测温β-GLYCOSIDASE TATβ-GLY
公开(公告)号：KR1020030088963A
公开(公告)日：2003-11-21
申请号：KR1020020026800
申请日：2002-05-15
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/56
Abstract: PURPOSE: Novel thermostable beta-glycosidase tat β-gly derived from Thermus flavus AT-62 is provided. The enzyme is easily purified because the protein derived from the mesophilic host cell can be easily removed by the simple heat treatment. CONSTITUTION: Novel thermostable beta-glycosidase tat β-gly derived from Thermus flavus AT-62 has the amino acid sequence of SEQ ID NO: 2, wherein the thermostable beta-glycosidase tat β-gly can maintain its activity at pH 7.0 and 70 deg. C for 70 hours or more, and has maximum enzyme activity at pH 5.0 to 6.0 and 80 to 90 deg. C. A gene encoding the amino acid sequence of SEQ ID NO: 2 has the nucleotide sequence of SEQ ID NO: 1.
Abstract translation: 目的：提供源自黄栖黄菌AT-62的新型热稳定性β-糖苷酶tat-Gly。 该酶易于纯化，因为通过简单的热处理可以容易地除去源自嗜温宿主细胞的蛋白质。 构成：源自Thermus flavus AT-62的新型热稳定性β-糖苷酶tat-Gly具有SEQ ID NO：2的氨基酸序列，其中耐热β-糖苷酶tatβ-gly可以在pH 7.0和70度维持其活性 。 70℃以上，并且在pH 5.0〜6.0和80〜90℃下具有最大的酶活性。 C.编码SEQ ID NO：2的氨基酸序列的基因具有SEQ ID NO：1的核苷酸序列。
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2.发明公开서머스 필리포미스(Ｔｈｅｒｍｕｓ ｆｉｌｉｆｏｒｍｉｓ)Ｗａｉ 33 Ａ１ 유래 신규의 내열성 베타-글리코시다제,ｔｆｉ β-ｇｌｙ 有权可从THERMUS FILIFORMIS WAI 33 A1中得到的热稳定β-谷氨酰胺酶TFIβ-GLY
公开(公告)号：KR1020030094518A
公开(公告)日：2003-12-18
申请号：KR1020020026801
申请日：2002-05-15
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/56
Abstract: PURPOSE: Thermostable beta-glycosidase tfi β-gly derived from Thermus filiformis Wai 33 A1 is provided. The enzyme is easily purified by the simple heat treatment, maintains its activity at pH 7.0 and 70 deg. C for 70 hours or more, and shows maximum enzyme activity at pH 5.0-6.0 and 80 to 90 deg. C. CONSTITUTION: A thermostable beta-glycosidase tfi β-gly derived from Thermus filiformis Wai 33 A1 has the amino acid sequence of SEQ ID NO: 6, wherein the thermostable beta-glycosidase tfi β-gly has improved lactose degrading activity; and the thermostable beta-glycosidase tfi β-gly is expressed from the recombinant Escherichia coli containing a gene encoding the thermostable beta-glycosidase tfi β-gly. The gene encoding the amino acid sequence of SEQ ID NO: 6 has the nucleotide sequence of SEQ ID NO: 5.
Abstract translation: 目的：提供源自Thermus filiformis Wai 33 A1的热稳定性β-糖苷酶tfiβ-gly。 酶易于通过简单的热处理纯化，其活性保持在pH 7.0和70度。 70小时以上，并且在pH 5.0-6.0和80至90度下显示出最大的酶活性。 C.构成：源自Thermus filiformis Wai 33A1的热稳定性β-糖苷酶tfiβ-gly具有SEQ ID NO：6的氨基酸序列，其中，所述耐热β-糖苷酶tfiβ-gly具有改善的乳糖降解活性; 并且由含有编码热稳定性β-糖苷酶tfiβ-gly的基因的重组大肠杆菌表达热稳定性β-糖苷酶tfiβ-gly。 编码SEQ ID NO：6的氨基酸序列的基因具有SEQ ID NO：5的核苷酸序列。
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3.发明公开서머스(Ｔｈｅｒｍｕｓ ｓｐ.) ＩＢ-21 유래 신규의내열성 베타-갈락토시다제, ｔｉｂ β-ｇａｌ 失效来自THERMUS SP的新型可热降解的β-GLYCOSIDASE TIBβ-GAL IB-21
公开(公告)号：KR1020030088964A
公开(公告)日：2003-11-21
申请号：KR1020020026802
申请日：2002-05-15
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/56
CPC classification number: C12N9/2471 , C12N15/70 , C12Y302/01023
Abstract: PURPOSE: Novel thermostable beta-glycosidase tib β-gal derived from Thermus sp. IB-21 is provided. The enzyme is easily purified because the protein derived from the mesophilic host cell can be easily removed by the simple heat treatment. CONSTITUTION: Novel thermostable beta-glycosidase tib β-gal derived from Thermus sp. IB-21 has the amino acid sequence of SEQ ID NO: 12, wherein the thermostable beta-glycosidase tib β-gal can maintain its activity at pH 7.0 and 70 deg. C for 70 hours or more, and has maximum enzyme activity at pH 5.0 to 6.0 and 80 to 90 deg. C. A gene encoding the amino acid sequence of SEQ ID NO: 12 has the nucleotide sequence of SEQ ID NO: 11. The thermostable beta-glycosidase tib β-gal is combined together in the form of homo-dimer.
Abstract translation: 目的：来自Thermus sp。的新型热稳定性β-糖苷酶tibβ-gal 提供IB-21。 该酶易于纯化，因为通过简单的热处理可以容易地除去源自嗜温宿主细胞的蛋白质。 构成：来自Thermus sp。的新型热稳定性β-糖苷酶tibβ-gal IB-21具有SEQ ID NO：12的氨基酸序列，其中热稳定的β-糖苷酶tibβ-gal可以在pH 7.0和70度维持其活性。 70℃以上，并且在pH 5.0〜6.0和80〜90℃下具有最大的酶活性。 C.编码SEQ ID NO：12的氨基酸序列的基因具有SEQ ID NO：11的核苷酸序列。将热稳定性β-糖苷酶tibβ-gal以同二聚体的形式组合在一起。
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4.发明授权서머스(Ｔｈｅｒｍｕｓ ｓｐ.) ＩＢ-21 유래 신규의내열성 베타-갈락토시다제, ｔｉｂ β-ｇａｌ 失效서머스（Thermus sp。）IB-21래래규규의베갈갈갈갈t t t t t t t t t tβββββββββ
公开(公告)号：KR100465134B1
公开(公告)日：2005-01-13
申请号：KR1020020026802
申请日：2002-05-15
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/56
Abstract: PURPOSE: Novel thermostable beta-glycosidase tib β-gal derived from Thermus sp. IB-21 is provided. The enzyme is easily purified because the protein derived from the mesophilic host cell can be easily removed by the simple heat treatment. CONSTITUTION: Novel thermostable beta-glycosidase tib β-gal derived from Thermus sp. IB-21 has the amino acid sequence of SEQ ID NO: 12, wherein the thermostable beta-glycosidase tib β-gal can maintain its activity at pH 7.0 and 70 deg. C for 70 hours or more, and has maximum enzyme activity at pH 5.0 to 6.0 and 80 to 90 deg. C. A gene encoding the amino acid sequence of SEQ ID NO: 12 has the nucleotide sequence of SEQ ID NO: 11. The thermostable beta-glycosidase tib β-gal is combined together in the form of homo-dimer.
Abstract translation: 用途：新型热稳定β-糖苷酶tib＆amp;β-gal来自Thermus sp。 提供IB-21。 该酶易于纯化，因为来自嗜温宿主细胞的蛋白质可以通过简单的热处理容易地除去。 构成：来自Thermus sp。的新型热稳定的β-糖苷酶tib＆amp;β; -gal。 IB-21具有SEQ ID NO：12的氨基酸序列，其中热稳定的β-糖苷酶tib＆amp;β-gal可以在pH7.0和70℃保持其活性。 70小时或更长时间，并且在pH 5.0至6.0和80至90℃下具有最大的酶活性。 编码SEQ ID NO：12的氨基酸序列的基因具有SEQ ID NO：11的核苷酸序列。热稳定的β-糖苷酶tib＆amp;β-gal以同二聚体的形式结合在一起。
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5.发明授权서머스 필리포미스(Ｔｈｅｒｍｕｓ ｆｉｌｉｆｏｒｍｉｓ)Ｗａｉ 33 Ａ１ 유래 신규의 내열성 베타-글리코시다제,ｔｆｉ β-ｇｌｙ 有权머포포포Therm Therm Therm Therm Therm Therm Therm Therm 33 33 33 33 33 Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm Therm
公开(公告)号：KR100465133B1
公开(公告)日：2005-01-13
申请号：KR1020020026801
申请日：2002-05-15
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/56
Abstract: PURPOSE: Thermostable beta-glycosidase tfi β-gly derived from Thermus filiformis Wai 33 A1 is provided. The enzyme is easily purified by the simple heat treatment, maintains its activity at pH 7.0 and 70 deg. C for 70 hours or more, and shows maximum enzyme activity at pH 5.0-6.0 and 80 to 90 deg. C. CONSTITUTION: A thermostable beta-glycosidase tfi β-gly derived from Thermus filiformis Wai 33 A1 has the amino acid sequence of SEQ ID NO: 6, wherein the thermostable beta-glycosidase tfi β-gly has improved lactose degrading activity; and the thermostable beta-glycosidase tfi β-gly is expressed from the recombinant Escherichia coli containing a gene encoding the thermostable beta-glycosidase tfi β-gly. The gene encoding the amino acid sequence of SEQ ID NO: 6 has the nucleotide sequence of SEQ ID NO: 5.
Abstract translation: 目的：提供来自Thermus filiformis Wai 33 A1的热稳定的β-糖苷酶tfi＆amp;β-gly。 该酶易于通过简单的热处理纯化，在pH 7.0和70℃下保持其活性。 70小时或更长时间，并且在pH 5.0-6.0和80-90℃显示最大的酶活性。 C.构成：来源于丝状栖热菌属（Thermus filiformis）外33A1的热稳定的β-糖苷酶tfi＆amp;β-gly具有SEQ ID NO：6的氨基酸序列，其中热稳定的β-糖苷酶tfiβ- 乳糖降解活性; 并且从含有编码热稳定的β-糖苷酶tfiβ-gly的基因的重组大肠杆菌中表达热稳定的β-糖苷酶tfiβ-gly。 编码SEQ ID NO：6的氨基酸序列的基因具有SEQ ID NO：5的核苷酸序列。
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