Abstract:
본 발명은 복제원점이 삽입된 원형(circular) DNA의 클로닝 방법에 관한 것으로, 복제원점이 없는 원형 DNA에 복제원점을 삽입하고 복제원점이 삽입된 원형 DNA를 대장균에 형질전환시켜 클로닝하는 방법을 제공한다. 본 발명의 클로닝 방법은 다양한 형태 및 크기의 원형 DNA에 적용이 가능하므로 다양한 원형 DNA를 클로닝할 수 있을 뿐만 아니라, 유전자 분석 및 조작에도 용이하게 이용될 수 있다.
Abstract:
A method for dispersing nanoparticles is provided to control array density of nanoparticles by adjusting pH of a charged nanoparticle dispersion, and thus form high-density nanoparticle single film without cohesion. A method for dispersing nanoparticles includes the steps of: (a) modifying the surfaces of nanoparticles to impart an electric charge to the surfaces of the nanoparticles; and (b) dispersing the surface-modified nanoparticles in a solvent and adjusting pH of the nanoparticle dispersion. A method for producing nanoparticle thin films comprises a step of arraying the pH-adjusted nanoparticle dispersion on a substrate by capillary force to prepare nanoparticle single film.
Abstract:
본 발명은 복제원점이 삽입된 원형(circular) DNA의 클로닝 방법에 관한 것으로, 복제원점이 없는 원형 DNA에 복제원점을 삽입하고 복제원점이 삽입된 원형 DNA를 대장균에 형질전환시켜 클로닝하는 방법을 제공한다. 본 발명의 클로닝 방법은 다양한 형태 및 크기의 원형 DNA에 적용이 가능하므로 다양한 원형 DNA를 클로닝할 수 있을 뿐만 아니라, 유전자 분석 및 조작에도 용이하게 이용될 수 있다.
Abstract:
A novel viral gene carrier is provided to deliver a gene specifically to tumor cells by controlling the gene delivery efficiency with low cytotoxicity and high stability and having higher transduction efficiency to a receptor positive KB cell. A viral gene carrier is characterized in that it is prepared by modifying the surface of a recombinant baculovirus vector including a green fluorescent protein, luciferase or a PDCD4 (programmed cell death 4) gene using a folate-polyethylene glcyol conjugate. A method for preparing the viral gene carrier comprises the steps of: (a) preparing the recombinant baculovirus vector; (b) preparing the folate-polyethylene glycol conjugate; and (c) modifying the recombinant baculovirus vector surface with the folate-polyethylene glycol conjugate.
Abstract:
A coexistence system for integrating heterogeneous wireless network devices, which use an identical frequency band, into a single board and an operation method thereof are provided to increase the throughput, reduce the frequency of retransmission, and minimize the power consumption by enabling an IEEE 802.11 WLAN and an IEEE 802.15.4 LR-WPAN to successfully execute their respective communication without any collision even though an identical frequency band is used. A coexistence system for integrating heterogeneous wireless network devices, which use an identical frequency band, into a single board comprises the first wireless network equipment(30), a synchronization unit(20), and the second wireless network equipment(10). The first wireless network equipment(30), or an IEEE 802.15.4 LR-WPAN, generates an interrupt to make a request for active channel reservation. The synchronization unit(20), such as ACROS(Active Channel Reservation for cOexiStence) software, forwards a channel reservation request interrupt in order to synchronize the time information between the first wireless network equipment(30) and the second wireless network equipment(10) which uses the same frequency band as the first wireless network equipment(30). The second wireless network equipment(10), or an IEEE 802.11 WLAN, reserves a radio channel through an RTS(Request To Send)/CTS(Clear To Send) switching scheme according to the channel reservation request interrupt. The second wireless network equipment(10), inactivated during the reservation period, helps the first wireless network equipment(30) to use the radio channel without interference.
Abstract:
본 발명은 신규한 바이러스성 유전자전달체에 관한 것으로, 폴레이트 리셉터를 표적할 수 있는 리간드로써 폴레이트를 폴리에틸렌 글리콜과 결합시켜 폴레이트-PEG(F-PEG)를 베큘로바이러스 표면에 도입하여 폴레이트 리셉터 매개의 세포이물흡수 (folate receptor-mediated endocytosis)를 유도하도록 함으로써 낮은 세포독성과 높은 안정성을 가지면서 유전자전달 효율을 조절할 수 있는 동시에 폴레이트 리셉터 파지티브 KB 세포에 훨씬 더 높은 트랜스덕션 효율을 가짐으로써 암세포에만 특이적으로 유전자를 전달할 수 있는 시스템을 구축할 수 있는 효과가 있다. 베큘로바이러스, 폴리에틸렌 글리콜, 폴레이트, 바이러스 벡터, 유전자전달체, 트랜스덕션