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公开(公告)号：KR100926096B1
公开(公告)日：2009-11-11
申请号：KR1020070098863
申请日：2007-10-01
Applicant: 재단법인서울대학교산학협력재단
IPC: A61K48/00 , C12N15/866 , A61K47/48 , A61P35/00
Abstract: 본 발명은 신규한 바이러스성 유전자전달체에 관한 것으로, 폴레이트 리셉터를 표적할 수 있는 리간드로써 폴레이트를 폴리에틸렌 글리콜과 결합시켜 폴레이트-PEG(F-PEG)를 베큘로바이러스 표면에 도입하여 폴레이트 리셉터 매개의 세포이물흡수 (folate receptor-mediated endocytosis)를 유도하도록 함으로써 낮은 세포독성과 높은 안정성을 가지면서 유전자전달 효율을 조절할 수 있는 동시에 폴레이트 리셉터 파지티브 KB 세포에 훨씬 더 높은 트랜스덕션 효율을 가짐으로써 암세포에만 특이적으로 유전자를 전달할 수 있는 시스템을 구축할 수 있는 효과가 있다.
베큘로바이러스, 폴리에틸렌 글리콜, 폴레이트, 바이러스 벡터, 유전자전달체, 트랜스덕션-
Patent Agency Ranking
公开(公告)号：KR1020090088184A
公开(公告)日：2009-08-19
申请号：KR1020080013577
申请日：2008-02-14
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: A61K9/1617 , A61K9/0053 , A61K9/113 , A61K9/1682 , A61K38/385
Abstract: A thiolated eutragit microsphere drug carrier for oral administration is provided to minimize the reduction of drug at low pH concentration and enhance the availability of drug. A method for manufacturing a thiolated eutragit microsphere drug carrier for oral administration comprises: a step of reacting L-cysteine hydrochloride with eutragit to obtain thiolated eutragit; a step of resolving thiolated eutragit in organic solvent to make thiolated eutragit solution; a step of resolving protein drug in distilled water to make drug solution; a step of adding protein drug solution in thiolated eutragit solution and treating with ultrasonic wave; a step of adding surfactant solution; and a step of solvent from water/oil/water emulsion and centrifuging.
Abstract translation: 提供用于口服给药的硫醇化微球药物载体以使药物在低pH浓度下的还原最小化并增加药物的可用性。 用于口服给药硫醇化微球药物载体的方法包括：使L-半胱氨酸盐酸盐与eutragit反应获得硫醇化的步骤; 在有机溶剂中分解硫醇化反应产生硫醇化溶液的步骤; 在蒸馏水中分解蛋白质药物制成药物溶液的步骤; 在硫醇化溶液中加入蛋白质药液并用超声波处理的步骤; 添加表面活性剂溶液的步骤; 和水/油/水乳液和离心的溶剂步骤。
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3.发明公开
公开(公告)号：KR1020080024016A
公开(公告)日：2008-03-17
申请号：KR1020060088277
申请日：2006-09-12
Applicant: 재단법인서울대학교산학협력재단
Abstract: A novel gene carrier is provided to reduce cytotoxicity and biodegradability, and improve gene deliver efficiency and complex-forming ability with DNA by using biodegradable polyesteramine based on polycaprolactone diacrylate and polyethyleneimine. A method for preparing a novel gene carrier comprises the steps of: independently dissolving polyethyleneimine and polycaprolactone diacrylate in anhydrous methyl alcohol; mixing the dissolved polyethyleneimine and polycaprolactone diacrylate; sealing the mixture with a solvent-resistant cover and reacting them at 40 deg. C for 24 hours; and subjecting the reaction product to dialysis with a membrane of 6,000-8,000 dalton at 4 deg. C for 24 hours. Further, a mol rate of the polycaprolactone diacrylate and the polyethyleneimine is 1:1 to 4.
Abstract translation: 提供了一种新的基因载体，通过使用基于聚己内酯二丙烯酸酯和聚乙烯亚胺的可生物降解的聚酯胺，降低细胞毒性和生物降解性，并提高DNA的基因传递效率和复合形成能力。 制备新基因载体的方法包括以下步骤：独立地将聚乙烯亚胺和聚己内酯二丙烯酸酯溶解在无水甲醇中; 混合溶解的聚乙烯亚胺和聚己内酯二丙烯酸酯; 用耐溶剂的盖子密封混合物，并将它们在40℃下反应。 C 24小时; 并用4,000-8,000道尔顿的膜在4℃下对该反应产物进行透析。 C 24小时。 此外，聚己内酯二丙烯酸酯和聚乙烯亚胺的摩尔比为1:1〜4。
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4.发明授权폴리카프로락톤 다이아크릴레이트와 폴리에틸렌이민을기초로 한 생분해성 폴리에스텔아민을 이용한 새로운유전자 전달체 失效一种基于聚己内酯二丙烯酸酯和聚乙烯亚胺作为基因载体的新型可生物降解聚酯胺
公开(公告)号：KR100860416B1
公开(公告)日：2008-09-26
申请号：KR1020060088277
申请日：2006-09-12
Applicant: 재단법인서울대학교산학협력재단
Abstract: 본 발명은 폴리카프로락톤 다이아크릴레이트와 폴리에틸렌이민을 기초로 한 생분해성 폴리에스텔아민으로 구성된 유전자 전달체에 관한 것이다. 더욱 상세하게는 본 발명은 폴리카프로락톤 다이아크릴레이트와 폴리에틸렌이민을 기초로 한 생분해성 폴리에스텔아민으로 구성된 유전자전달체로써 낮은 세포독성, 생분해성 및 높은 유전자 전달효율로 유전자를 효율적으로 전달하는 뛰어난 효과가 있다.
유전자전달체, 생분해성, 폴리에스텔아민, 폴리카프로락톤 다이아크릴레이트, 폴리에틸렌이민-
5.发明公开피에이치 감응성과 점막점착성을 갖는 유드라지트로 코팅된단백질 경구투여용 키토산미립자 및 그 제조방법 无效用于通过敏感和不饱和脂肪族螺旋体衍生物涂覆的口服蛋白质药物递送的壳聚糖微球及其制备方法
公开(公告)号：KR1020090039513A
公开(公告)日：2009-04-22
申请号：KR1020070105205
申请日：2007-10-18
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: C08B37/003 , A61K9/0053 , A61K9/167 , A61K47/36
Abstract: A chitosan particle coated with thiolated eudragit, in which the BSA(Bovine Serum Albumin) is soaked, is provide to use as a delivery agent of a drug by oral administration. A manufacturing method of a chitosan particulate for a protein oral administration coated with eudragit having a pH sensitivity and mucous adhesive property comprises: a step of sonicating and centrifuging the chitosan solution to obtain chitosan particle; a step of adding BSA to the chiotsan particle, suspending to distribute the chitosan particle, and stirring at 35-40°C for 12-24 hours to soak the BSA into chitosan particle; and a step of adding the thiolated eudragit solution to the BSA-soaked chitosan particle, stirring and centrifuging to obtain the chitosan particle coated with BSA-dipped thiolated eudragit. The addition rate of the thiolated eudragit solution to the BSA-dipped chitosan particle is 1:1-1:4.
Abstract translation: 将BSA（牛血清白蛋白）浸泡的硫蛋白包被的壳聚糖颗粒通过口服给药用作药物的递送剂。 用于具有pH敏感性和粘性粘合性质的用于蛋白质口服给药的壳聚糖颗粒的制造方法包括：超声处理和离心壳聚糖溶液以获得壳聚糖颗粒的步骤; 将BSA加入到悬浮颗粒中，悬浮以分散壳聚糖颗粒，并在35-40℃下搅拌12-24小时以将BSA浸泡到壳聚糖颗粒中; 将硫醇化的eudragit溶液加入到BSA-浸泡的壳聚糖颗粒中，搅拌和离心以获得涂布有BSA-浸渍的硫苷酸脱乙酰壳多糖的壳聚糖颗粒的步骤。 硫苷酸脱乙酰壳多糖溶液对BSA-浸渍壳聚糖颗粒的添加速率为1:1-1:4。
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公开(公告)号：KR1020080029946A
公开(公告)日：2008-04-03
申请号：KR1020070098863
申请日：2007-10-01
Applicant: 재단법인서울대학교산학협력재단
IPC: A61K48/00 , C12N15/866 , A61K47/48 , A61P35/00
CPC classification number: A61K48/00 , A23V2200/308 , C12N15/866
Abstract: A novel viral gene carrier is provided to deliver a gene specifically to tumor cells by controlling the gene delivery efficiency with low cytotoxicity and high stability and having higher transduction efficiency to a receptor positive KB cell. A viral gene carrier is characterized in that it is prepared by modifying the surface of a recombinant baculovirus vector including a green fluorescent protein, luciferase or a PDCD4 (programmed cell death 4) gene using a folate-polyethylene glcyol conjugate. A method for preparing the viral gene carrier comprises the steps of: (a) preparing the recombinant baculovirus vector; (b) preparing the folate-polyethylene glycol conjugate; and (c) modifying the recombinant baculovirus vector surface with the folate-polyethylene glycol conjugate.
Abstract translation: 提供了一种新的病毒基因载体，通过以低细胞毒性和高稳定性控制基因传递效率并且对受体阳性KB细胞具有较高的转导效率，特异性地向肿瘤细胞递送基因。 病毒基因载体的特征在于其通过使用叶酸 - 聚乙烯胶体缀合物修饰包含绿色荧光蛋白，荧光素酶或PDCD4（程序性细胞死亡4）基因的重组杆状病毒载体的表面来制备。 制备病毒基因载体的方法包括以下步骤：（a）制备重组杆状病毒载体; （b）制备叶酸 - 聚乙二醇缀合物; 和（c）用叶酸 - 聚乙二醇缀合物修饰重组杆状病毒载体表面。
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