公开(公告)号：KR1020090093166A

公开(公告)日：2009-09-02

申请号：KR1020080018534

申请日：2008-02-28

Applicant: 전남대학교산학협력단

Inventor： 윤철호 ,  임성근 ,  김동현 ,  김건희

IPC: C12Q1/32 ,  C12Q1/26 ,  G01N33/52 ,  G01N33/50

CPC classification number: C12Q1/32 ,  G01N2333/90209 ,  C12Y106/02004 ,  G01N33/52

Abstract: A method for measuring the activation of NADPH-cytochrome P450 reductase (CPR) in a sample with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is provided to shorten the analysis time and easily and accurately detect a small amount of CTC-formazan. A method for measuring the activation of NADPH-cytochrome P450 reductase (CPR) in a sample comprises: a step of reacting a sample containing an enzyme having CPR activation with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) under the presence of NADPH to convert the CTC to CTC-formazan; and a step of measuring the concentration of CTC-formazan with spectrophotometric or spectrofluorometric method.

Abstract translation: 提供了一种用5-氰基-2,3-二苯基四唑氯化物（CTC）测定样品中NADPH-细胞色素P450还原酶（CPR）活化的方法，以缩短分析时间，容易且准确地检测少量CTC -formazan。 用于测量样品中NADPH-细胞色素P450还原酶（CPR）的活化的方法包括：将含有CPR活化的酶的样品与5-氰基-2,3-二甲苯基四唑氯化物（CTC）反应的步骤， 的NADPH将CTC转化为CTC-甲an; 并用分光光度法或分光荧光法测定CTC-甲the的浓度。